human bladder cancer cell line umuc3  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Fluorescence Enhancement Effect of TiO 2 Nanoparticles and Application for Photodynamic Diagnosis

doi: 10.3390/ijms20153698

Figure Lengend Snippet: Fluorescence microscopic imaging of UMUC3 cells at 30 min after administration of fluorescently labeled TiO 2 -PEG NPs: blue indicates nuclei, green indicates fluorescently labeled TiO 2 -PEG NPs ( Left ) at 100× magnification; ( Right ) 3D imaging at 630× magnification.

Article Snippet: The UMUC3 human bladder cancer cell line was purchased from American Type Culture Collection (ATCC) (CRL-1749, Rockville, MD, USA).

Techniques: Fluorescence, Imaging, Labeling

Journal: International Journal of Molecular Sciences

Article Title: Fluorescence Enhancement Effect of TiO 2 Nanoparticles and Application for Photodynamic Diagnosis

doi: 10.3390/ijms20153698

Figure Lengend Snippet: Analysis of endocytosis by UMUC3 cells after administration of fluorescently labeled TiO 2 -PEG NPs: ( A ) Immunostaining images of early endosomes using an anti-EEA1 antibody at 630× magnification, blue indicates nuclei, green indicates fluorescently labeled TiO 2 -PEG NPs, red indicates immunostained endosomes (upper) late endosome stained using an anti-LAMP1 antibody (lower); ( B ) Colocalization between endosomes and TiO 2 -PEG NPs ( n = 3, error bar mean + SD); ( C ) Intracellular concentration of TiO 2 -PEG NPs ( n = 3, error bar mean + SD).

Article Snippet: The UMUC3 human bladder cancer cell line was purchased from American Type Culture Collection (ATCC) (CRL-1749, Rockville, MD, USA).

Techniques: Labeling, Immunostaining, Staining, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Fluorescence Enhancement Effect of TiO 2 Nanoparticles and Application for Photodynamic Diagnosis

doi: 10.3390/ijms20153698

Figure Lengend Snippet: Measurement of the fluorescence spectrum in UMUC3 cells after combined administration of TiO 2 -PEG NPs and ALA: combined administration of TiO 2 -PEG NPs and ALA indicated as “NPs and ALA treatment”, administration of ALA indicated as “ALA alone”, ( A ) fluorescence microscopic imaging of UMUC3 cells at 630× magnification, administration of ALA alone ((i), upper), combined administration of TiO 2 -PEG NPs and ALA after 2 h of TiO 2 -PEG NPs treatment ((ii), lower); ( B ) Spectrum intensity of UMUC3 cells after administration of ALA alone, or combined administration of TiO 2 -PEG NPs and ALA after 2 of TiO 2 -PEG NPs treatment; ( C ) Time course of fluorescence intensity during irradiation; ( D ) Evaluation of fluorescence ratio comparing the administration of ALA alone with treatment time of TiO 2 -PEG NPs; ( E ) Intracellular amount of PpIX ( n = 3, error bar mean ± SD, * p ≤ 0.05, ** p ≤ 0.01) .

Article Snippet: The UMUC3 human bladder cancer cell line was purchased from American Type Culture Collection (ATCC) (CRL-1749, Rockville, MD, USA).

Techniques: Fluorescence, Imaging, Irradiation

Journal: OncoTargets and therapy

Article Title: Norcantharidin inhibits the DDR of bladder cancer stem-like cells through cdc6 degradation

doi: 10.2147/OTT.S209907

Figure Lengend Snippet: Characterization of BCSLCs. ( A, B ) Expression of CD34, CD44, ABCG2, CD133, OCT4 and NANOG in UMUC3 cells and BCSLCs according to qPCR assays. ( C ) Flow cytometry was used to detect CD133+ cells in UMUC3 cells and BCSLCs. ( D ) Proportion of CD133+ cells (%) is presented. ( E ) Cell gaps were photographed at 0, 24, 48, and 72 hr after scratching. ( F ) Distance between cells in the scratch zone (% of basal) is shown. ( G ) Cell migration and invasion as determined by the transwell assay were photographed. ( H ) Cells per field were counted. ( I ) Subcutaneous tumor formation was established under the skin on the side of the back in nude mice with 5×10 6 cells UMUC3 cells and BCSLCs after 4 weeks (Left/UMUC3 cells, Right/BCSLCs). ( J ) The tumors were extracted from the mice after day 28 (Upper/BCSLCs, Lower/UMUC3 cells). There were significant differences in size. (* P <0.05). Abbreviation: BCSLCs, bladder cancer stem-like cells.

Article Snippet: Cells from human bladder cancer cells line UMUC3 (obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA)) were cultured through the standard procedure in RPMI-1640 (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco), 1% penicillin and 1% streptomycin (Gibco) under standard conditions (5% CO2 and 95% atmosphere, 37 °C).

Techniques: Expressing, Flow Cytometry, Migration, Transwell Assay

Journal: OncoTargets and therapy

Article Title: Norcantharidin inhibits the DDR of bladder cancer stem-like cells through cdc6 degradation

doi: 10.2147/OTT.S209907

Figure Lengend Snippet: Tumor formation rate from cells injected at different concentrations

Article Snippet: Cells from human bladder cancer cells line UMUC3 (obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA)) were cultured through the standard procedure in RPMI-1640 (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco), 1% penicillin and 1% streptomycin (Gibco) under standard conditions (5% CO2 and 95% atmosphere, 37 °C).

Techniques: Injection

Journal: OncoTargets and therapy

Article Title: Norcantharidin inhibits the DDR of bladder cancer stem-like cells through cdc6 degradation

doi: 10.2147/OTT.S209907

Figure Lengend Snippet: Volume of subcutaneous tumors in the UMUC3 and BCSLCs groups of mice (mean ± SD) (n=5) (* P <0.05)

Article Snippet: Cells from human bladder cancer cells line UMUC3 (obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA)) were cultured through the standard procedure in RPMI-1640 (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco), 1% penicillin and 1% streptomycin (Gibco) under standard conditions (5% CO2 and 95% atmosphere, 37 °C).

Techniques:

Journal: OncoTargets and therapy

Article Title: Norcantharidin inhibits the DDR of bladder cancer stem-like cells through cdc6 degradation

doi: 10.2147/OTT.S209907

Figure Lengend Snippet: The half maximal inhibitory concentration (IC50) of chemotherapy drugs to UMUC3 and BCSLCs (mean ± SD) (n=3) (* P <0.05)

Article Snippet: Cells from human bladder cancer cells line UMUC3 (obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA)) were cultured through the standard procedure in RPMI-1640 (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco), 1% penicillin and 1% streptomycin (Gibco) under standard conditions (5% CO2 and 95% atmosphere, 37 °C).

Techniques: Concentration Assay

Journal: OncoTargets and therapy

Article Title: Norcantharidin inhibits the DDR of bladder cancer stem-like cells through cdc6 degradation

doi: 10.2147/OTT.S209907

Figure Lengend Snippet: The effect of NCTD on BCSLCs in vitro. ( A ) The protein expression of Cdc6-C in BCSLCs after NCTD treatment for 48 hr (0 μM, 50 μM, 100 μM and 200 μM) was detected by Western blotting. ( B ) The relative OD for Cdc6-C was determined from the Western blot band. ( C ) The expression of ATR-C, pChk1, pCdc25C in BCSLCs and UMUC3 cells after treatment with or without NCTD (50 μM) was detected by Western blotting. ( D – F) The relative OD data for ATR-C, pChk1, pCdc25C was determined from the Western blot band. ( G ) Relative cell viability of different groups is shown. (* P <0.05). Abbreviations: BCSLCs, bladder cancer stem-like cells; Cdc6-C, chromatin-binding Cdc6; NCTD, norcantharidin; ATR-C, Chromatin-binding ATR; pChk1, phosphorylated check point kinase 1; pCdc25C, phosphorylated Cdc25C.

Article Snippet: Cells from human bladder cancer cells line UMUC3 (obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA)) were cultured through the standard procedure in RPMI-1640 (Gibco, Grand Island, NY, USA) with 10% fetal bovine serum (Gibco), 1% penicillin and 1% streptomycin (Gibco) under standard conditions (5% CO2 and 95% atmosphere, 37 °C).

Techniques: In Vitro, Expressing, Western Blot, Binding Assay